Identifying Two Unknown Species of Bacteria Essay -- Escherichia Coli

Identifying devil foreigner Species of bacteriumMaterials and Methods week 1, solar day 1 (10 November 2000)The premier(prenominal) day an unmapped try on was designate to for individu tout ensembley unrivaled concourse of students. The offshoot riddle utilise was a gigabyte speckle to scrutiny for g-force exacting or Gram- detrimental bacteria. The word structure of the both(prenominal) types of bacterium was viewed nether the microscope and recorded. thus the seek was limit on nutrient agar-agar ho rehearses utilise the quarter-circle mottle method for closing off. at that place were ternion agar plates one was incubated at elbow live temperature, the entropy at 30 degrees Celsius, and the leash at 37 degrees Celsius. By placing individually plate at a diametric temperature optimal step-up temperature domiciliate be predicted for both species of bacterium. workweek 1, twenty- four hours 2 (12 November 2000) afterward 48 hour s of incubation the agar plates were viewed. exclusive colonies were tried and true for prospered closing off by gibibyte dye and and so think the dye bacterium below a microscope. isolation was successful. i dependency of from for each one one recondite bacterium was transferred to an agar topple for enhanceth. The agar bloods were stored at room temperature everyplace the spend so that they would non arouse besides much. week 2, sidereal day 1 (17 November 2000) later 5 eld of emersion each slant was essayed victimization the thousand staining technique to fend for the nab isolation of the bacteria. twain isolations were all told successful. thusly each experiment of bacteria was subjected to a series of political campaignings for identification. adept bacterium was gm negative. It underwent four polar analyses. These tests were the EMB test (eosin Mehylene gloomy), the reciprocal ohm Indole app arent movement (SIM) test, t he Urease test, and the Simmons citrate consumption test. The EMB test checks for a bacterias qualification to do work milk sugar. This test is action by placing the bacteria on Eosin methylene Blue agar. The agar is discriminating for thousand negative bacteria and those bacteria that provoke acidulate lactose forget founder change growth, comm only when a gold-bearing parkland sheen. The sec Indole apparent motion agar tests for cardinal get out characteristics entropy reduction, indole production, and question. The SIM specialty is a solid long suit this facilitates the motility test. The long suit contains sulfur, if the bacterium has the business leader to annul sulfur the ordinary leave alone wind black. The fair in any case contains tryptophan. If the bacterium has the enzyme tryptophanase, indole leave behind be ... ...indole, it is motile, in that respect is no urease look and there is no coagulase activity. By conditional relation and formal reason out inexplic fitting 10a was heady to be Escherichia coli. foreigner 10b is staphylococci epidermidis. concord to Bergeys manual of arms(a) staphylococci bacteria are universal gravitational constant controlling orbiculate cells that devolve singly, in pairs or in bit clusters. unvalued 10b was gram positive, world(a) and occurred in clusters. Bergeys manual(a) also says the bacteria grow substantially in mettlesome sodium chloride concentrations. foreign 10b grew hygienic on the mannitol salt agar. The optimum growing temperature is 30-37 degrees Celsius (Bergeys manual of arms). mystical 10b grew outgo at 37 degrees Celsius. The testing ground manual and medieval(a) lab results corroborate all other test results. inscrutable 10b was only able to use da Gamma lysis, it was ineffective to fermentation mannitol and had no coagulase activity. When compare to past labs it is substantiate that nameless 10b is staph epidermidis. unvalued 10 contained 2 bacteria they are Escherichia coli and staphylococci epidermidis. References1.Holt, magic trick G. et al Bergeys Manual of determinant Bacteriology, 1994.2.Merkel, Brian Microbiology Laboratory, 2000.